* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.
Gene silencing refers to the phenomenon that biological cells can inhibit the expression of a gene using various gene expression regulation mechanisms. There are two main types of gene silencing.
Gene silencing at the transcriptional level mainly includes DNA methylation, location effects, genomic imprinting, gene transfer, repeat-induced point mutations, and paramutation.
Post-transcriptional gene silencing mainly includes co-inhibition and RNA silencing (including RNA interference, gene quelling, and unpaired DNA-mediated meiosis silencing, guided by small RNAs such as microRNA (miRNA), small interfering RNAs (siRNAs), Piwi-associated RNAs (piRNAs), and mRNA degradation, etc. For example, the 3' untranslated region (3'UTR) of many mRNAs can bind to miRNA to reduce mRNA translation and achieve gene silencing.
In biological research, to study the function of a gene, researchers will use RNA interference or CRISPR gene-knocking technology to achieve gene silencing, greatly reduce the performance of the target gene to detect its influence, and then infer the function of the target gene. Compared with the expression of target genes completely removed by gene deletion, although the expression of target genes was inhibited by gene knocking, it still retained a certain degree of expression.
In conclusion, gene silencing is an important way of gene expression regulation and a self-protection mechanism for organisms at the level of gene regulation. It is common in foreign DNA invasion, virus infection, DNA transposition, and rearrangement. An in-depth study of gene silencing can help researchers to further reveal the nature of gene expression regulation of organisms, and overcome the phenomenon of gene silencing so that exogenous genes can be more effectively expressed according to people's needs. Gene silencing can effectively inhibit the expression of harmful genes in gene therapy to achieve the purpose of disease treatment, so the study of gene silencing has extremely important theoretical and practical significance.
|Catalog No.||Product Name||Description|
|BT-000001||DNA in vivo Delivery Kit||Reagents designed for plasmid DNA in vivo transfection.|
|BT-000044||siRNA/DNA Transfection Reagent||BOC Sciences' siRNA/DNA transfection reagent is a highly efficient, low toxicity, ideal siRNA and plasmid DNA transfection reagent for mammalian cells.|
|BT-000030||DOTAP-Lipo Transfection Reagent||Reagents for DNA, RNA and protein transfection.|
|BT-000023||Broad Spectrum DNA Transfection Reagent||Reagents for DNA transfection.|
|BT-000046||CRISPR RNP Transfection Reagent||BOC Sciences' CRISPR RNP transfection reagent is a polymer-based transfection reagent with a membrane fusion virus mechanism that can efficiently deliver Cas9 ribonucleoprotein (RNP) in vitro.|
|BT-000005||Cas9 Transfection Reagent||Optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 protein delivery|
|BT-000042||HTT mRNA Transfection Reagent||Reagents for high-efficiency delivery of mRNA into hard-to-transfect cells.|
|BT-000041||mRNA Transfection Reagent||Transfection reagent for mRNA.|
|BT-000043||siRNA Transfection Reagent||BOC Sciences' siRNA transfection reagent is an efficient, low toxicity, ideal siRNA transfection system for mammalian cells.|
|BT-000012||Hela CRISPR Transfection Reagent||Proprietary preparation reagent optimized for delivery of CRISPR/Cas9 gene modification system into Hela cells.|
|BT-000014||HepG2 CRISPR Transfection Reagent||Proprietary preparation reagent optimized for delivery of CRISPR/Cas9 gene modification system into HepG2 cell line.|
|BT-000021||Neuro-2a Transfection Reagent||Reagents designed for efficient delivery of DNA and RNA into neuroblastoma cells.|
|BT-000020||Neuro-Transfection Reagent||Reagents designed for high-efficient transfection of DNA into nerve cells.|