* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.
RNA transfection technology is the process of introducing RNA molecules such as mRNA, siRNA and viral RNA into cells and expressing them. RNA transfection techniques are mainly used to study the function of RNA, where transfection of mRNA has been successful in short-term protein expression, virus production and replication studies.
Since they are all nucleic acids, traditional DNA transfection methods can be used to transfect RNA, but the biggest headache is the easy degradation of RNA and the prevention of RNAse contamination.
An additional note in transfection is that transfection reagents should be confirmed free of RNase contamination and also try to avoid mixing with plasmid DNA transfection - even if the reagents themselves are compatible with DNA and RNA transfection. So RNA-specific transfection reagents would be better. It is also important to use a good quality serum that is free of RNAse contamination.
The amount of mRNA transfected must be referred to the transfection reagent instructions, as the ratio of mRNA to transfection reagent determines the structure and net charge of the transfection complex and whether it is readily adsorbed and endocytosed by the cell membrane.
In addition to manipulation, the structure of the RNA itself also has an influence on transfection.
Mammalian mRNA molecules have a 5' end cap structure and a 3' end Poly (A) tail, in addition to ribosomal binding regions called IRES (internal ribosomal entry site), which either contribute to the stability of the mRNA or help ribosomes bind to mRNA for translation. For RNA obtained by in vitro transcription, this protective "cap" can be obtained by experimentally adding analogs of the cap structure at the 5' end of RNA, and the 3' end Poly (A) tail can also be experimentally added. Adding these elements in most cases helps to improve the stability of the RNA once it enters the cell. However, sometimes the IRES element promotes translation, and in some cell lines, due to the lack of the corresponding binding protein, the IRES affects transcription.
|Product Name||mRNA Transfection Reagent|
|Shelf Life||1 year, if stored and handled correctly.|
|Storage||Store at 4°C.|
|Characteristics||Shorter mRNA has higher transfection efficiency.|
mRNA is used directly in the translation process to produce proteins faster and with higher productivity.
mRNA has no risk of genomic integration and has higher safety.
|Product Name||HTT mRNA Transfection Reagent|
|Shelf Life||6 months.|
|Storage||Store at 4°C.|
|Characteristics||No risk of genome integration.|
Very suitable for CRISPR/Cas9 gene editing, iPS generation, stem cell differentiation, and immunotherapy assays.
Unmatched transfection efficiency (including a variety of cells that are difficult to transfect).
Low cytotoxicity, extremely gentle on cells.