* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.
|BT-000040||Large RNA Transfection Reagent||Inquiry|
Transfection is the process of introducing DNA, RNA or proteins into eukaryotic cells for the study and regulation of gene expression. Find large RNA transfection Kits with low toxicity at BOC Sciences!
RNA is a chain molecule formed by ribonucleotides through phosphodiester bonds. According to the length of the RNA strand, RNA is divided into small RNA and long RNA. Typically, small RNAs are less than 200 nt in length, while long RNAs are greater than 200 nt in length. Long-chain RNAs, also known as large RNAs, mainly include long-chain non-coding RNAs (lncRNAs) and mRNAs.
mRNA, messenger ribonucleic acid, is a type of single-stranded ribonucleic acid that is transcribed from a strand of DNA as a template and carries genetic information that can guide protein synthesis. lncRNAs are no-coding transcripts longer than 200 nucleotides. Most lncRNAs are also transcribed by RNA polymerase II, but their sequence conservation is not high and their expression abundance is low, showing strong specificity in tissues and cells. According to their position with adjacent genes, they can be divided into antisense lncRNA, sense lncRNA, intergenic lncRNA, bidirectional lncRNA, and intronic lncRNA. The number of lncRNAs not only dominates ncRNAs (non-coding RNAs), but also has multiple biological functions, such as acting as molecular scaffolds in the nucleus, assisting in alternative splicing, regulating chromosome structure, or regulating translation in the cytoplasm, promoting or inhibiting mRNA degradation, adsorbing miRNA (as ceRNA), etc., are also involved in the regulation of cell differentiation and ontogeny at multiple levels, and are closely related to diseases.
Suitable for virus production, protein expression, and CRISPR genome editing