* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

CHO cells exhibit epithelioid cell morphology and are widely used in biomedical research and biotechnology R&D applications. BOC Sciences CHO Transfection Reagent is a unique reagent that has been specially optimized to provide excellent transfection efficiency in CHO cells and related lineage cell types.

Introduction of CHO cells

CHO cells were obtained from Chinese hamster ovary cells in 1957. They belong to fibroblasts, which are non-secreting cells. They rarely secrete CHO endogenous proteins, so they are very beneficial for the isolation and purification of target proteins. They can form active dimers (such as interleukin 2) and have glycosylation functions (such as EPO), and CHO is an ideal host for the expression of complex biological macromolecules. CHO-K1 cells are widely used in industrial production. They have transformed cell lines and are widely used to express recombinant DNA proteins.

CHO Transfection Kits

Optimizing Transfection

• For the highest transfection efficiency, optimize the transfection conditions by varying the CHO/CHO-K1 cell density and the amount of transfection reagent.
• High passage of CHO/CHO-K1 cells and use of antibiotics (or growth factors) may require using larger volumes of CHO/CHO-K1 transfection reagent per reaction.

Highlights

• Liposome-based lipid formulations
• Easy-to-use transfection protocol with reproducible results
• Low cytotoxicity
• Efficient delivery

Transfection Methods

Transfection methods of CHO cells are divided into transient transfection and stable transfection.

• Transient transfection is the introduction of recombinant DNA into a highly infectious cell line to obtain temporary but a high-level expression of the gene of interest. Transfected DNA does not have to be integrated into the host chromosome, and transfected cells can be harvested in a shorter time than stable transfection.
• Stable transfection is the integration of exogenous genes into the cell's genome. As the cells grow and divide, the exogenous genes can be stably transfected and expressed. Meanwhile, after screening by antibiotic stress, cell lines that can stably transfect and express proteins are finally obtained.

Fig. 1 Schematic diagrams of two different transfections (Kim TK, 2010)

Reference

1. Kim TK; et al. Mammalian cell transfection: the present and the future. Anal Bioanal Chem. 2010 Aug; 397(8): 3173-8.

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