* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.
| Catalog | Product Name | Inquiry |
|---|---|---|
| BT-000030 | DOTAP-Lipo Transfection Reagent | Inquiry |
| BT-000046 | CRISPR RNP Transfection Reagent | Inquiry |
CRISPR RNP transfection kit including DOTAP-Lipo transfection reagent and CRISPR RNP transfection reagent. Find CRISPR RNP transfection Kits with low toxicity at BOC Sciences!
CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats, is a repetitive DNA structure accidentally discovered in bacterial DNA by Japanese scientist Yoshino Ishino in 1987. It is a means for bacteria to identify and protect themselves from viral DNA invasion.
The third-generation editing technology developed based on this sequence, CRISPR-cas9 technology relies on two key parts: the Cas9 protein, which acts as a "wrench," and the CRISPR guide RNA (gRNA), which acts as a "screw". The "screw" is responsible for locating the target site and recruiting and activating the Cas9 protein, the Cas9 protein is responsible for cutting DNA. This editing technology uses the Cas9 enzyme, which cuts small RNA (guide RNA, gRNA) into DNA as a guidance tool where cleavage or other gene editing can be performed. There is no doubt that CRISPR-Cas9 is a powerful tool that makes gene editing faster, more accurate, cheaper, and easier to operate, and can also be used in many applications, including human medicine, agriculture, and biofuels, etc.
There are various delivery systems in the CRISPR system. The RNP (Ribonucleoprotein complex) delivery system directly uses crRNA: tracrRNA or single-stranded sgRNA to locate the Cas9 endonuclease to the target sequence, which is a delivery system with high editing efficiency and low off-target effect. Meanwhile, the operation is simple and fast, effectively saving time and labor costs. RNP is a complex with editing function formed by the combination of Cas9 protein and gRNA. Compared with other CRISPR/Cas9 methods, RNP has low cytotoxicity, is not easy to induce immune responses, and can cut DNA fragments in vitro.
| Products | Cell Types | Application |
|---|---|---|
| Neuro-Transfection Reagent | Primary nerve cells, glial cells, and cultured nerve cell lines |
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| Neuro-2a Transfection Reagent | Neuroblastoma cells | |
| Astrocytes Transfection Reagent | Astrocytes cells |
