Nucleic Acid Co-transfection

* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

Co-transfection refers to the simultaneous transfection of two separate nucleic acid molecules (such as plasmid DNA and siRNA). Transient co-transfection of multiple plasmid DNAs is a technique increasingly adopted by biological researchers, and is often used in cell-protein interaction studies using shRNA-encoding plasmids, transcription factor studies, and gene knockout studies.

Common examples of nucleic acid co-transfection are as follows:

  • Transient co-transfection of multiple plasmid DNAs (can be used for overexpression of different DNAs, functional studies between different genes, co-delivery for virus production, etc.).
  • Co-transfection of plasmid DNA and siRNA-for gene function research.
  • Co-delivery of plasmid DNA and mRNA for gene insertion (e.g. CRISPR/Cas KI).

Tips for nucleic acid co-transfection

  • The ratio of nucleic acids
  • Most transfection reagents are designed to effectively deliver a single nucleic acid type, such as plasmid DNA, siRNA, mRNA, etc. Therefore, it is relatively simple to co-transfect multiple nucleic acids of the same kind (such as multiple plasmid DNA or siRNA), as long as the ratio of transfection reagent to total nucleic acid is maintained. Co-delivery of completely different nucleic acid types can be very tricky. Mainly due to the size and charge differences between these nucleic acid molecules, the co-transfection of different nucleic acid types (such as large plasmid DNA and small siRNA/miRNA) can be challenging.

    In order to obtain the best experimental results, it may be necessary to further optimize the ratio of each plasmid according to the experimental purpose, the promoter element of each plasmid and the protein to be expressed.

  • Premix of nucleic acid
  • It is also important to prevent one plasmid DNA from complexing with the transfection reagent in preference to another. This can be done by completely premixing the plasmid DNA before adding the transfection reagent. If the plasmid mixture is completely mixed before forming the transfection complex, the order of adding different plasmid DNA will not affect the transfection result.

    If a single transfection reagent is not suitable for a specific co-delivery application, the best transfection reagent for each nucleic acid can be used to prepare separate transfection complexes and add them to the cells at the same time. As long as the various transfection protocols are compatible, this method can ensure that the cells take up all the nucleic acids at the same time.

Co-transfection Solutions

  • BS-Plasmid DNA Transfection Reagent (BT-000026)
  • BS-Plasmid DNA transfection reagent adopts our most advanced liposome technology, which can promote the efficient delivery of plasmid DNA to mammalian cells, and has excellent transfection performance and low cytotoxicity. It can provide excellent transfection efficiency for various common types of cells and cells that are difficult -to -transfect, and provide reproducible experimental results.

  • DOTAP-Lipo Transfection Reagent (BT-000030)
  • DOTAP-Lipo transfection reagent uses a proprietary cationic lipid formula, which can effectively deliver negatively-charged molecules, including DNA, RNA, oligonucleotides, and ribonucleoprotein (RNP) complexes. It can achieve excellent transfection efficiency in different types of cells (such as insect cells, mammalian cells) and improve cell viability.

  • siRNA/DNA Transfection Reagent (BT-000044)
  • siRNA/DNA transfection reagent is an efficient, low-toxicity, ideal siRNA and plasmid DNA transfection reagent for various mammalian cells, and is very suitable for siRNA/DNA co-transfection.