Transient Transfection

* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

What is transient transfection?

In transient transfection, the introduced nucleic acid only exists in the cell for a limited period and is not integrated into the genome. In this way, the transiently transfected genetic material will not be transferred from generation to generation during cell division and may be lost due to environmental factors or diluted during cell division. However, in a short period of time, the high copy number of foreign genes in the cell nucleus continued to be expressed, resulting in increased levels of protein expression within the cell.

Cells are usually collected 24-72 hours after transfection for research aimed at analyzing the transient expression of transfected genes. The optimal time interval depends on the cell type of the transferred gene, research objectives, and specific expression characteristics. Analysis of gene products may require isolation of RNA or protein for enzyme activity assays or immunoassays, and the method used to harvest the cells will depend on the final product analyzed.

Your instant transfection partner

We provide an unparalleled variety of transfection reagents to meet your different needs. For researchers who wish to establish transient protein production in mammalian and insect cells in their laboratories, we provide variety types of transient transfection reagents to meet your different needs.

Tips for transient transfection

When performing transient transfection, you can choose standard or reverse transfection protocols.

  • In the standard transfection protocol, cells are plated on day 1, transfected on day 2, and assayed on days 3 or 4.
  • In the reverse transfection protocol, cells are added directly to the plate containing the transfection reagent/DNA mixture, and assay 2 or 3 is performed on the first day.
  • Since the cells are added directly to the DNA, this process reduces the experimental time by one day and allows the high-throughput transfection of DNA in a plate or microarray format.

Transient Transfection VS Stable Transfection

  • Transient Transfection

    • The transfected nucleic acid stays in the cell for a limited period.
    • The transfected material gets lost during cell division.
    • Transfection effects can be observed normally between 1-7 days after transfection.
    • Quick and easy.
  • Stable Transfection

    • The transfected material is integrated into the genome.
    • Uptake of foreigner DNA material into the genome requires optimal transfection conditions and selection of positive cells.
    • Experiment needs approx. 1 month.