* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.
In transient transfection, the introduced nucleic acid only exists in the cell for a limited period and is not integrated into the genome. In this way, the transiently transfected genetic material will not be transferred from generation to generation during cell division and may be lost due to environmental factors or diluted during cell division. However, in a short period of time, the high copy number of foreign genes in the cell nucleus continued to be expressed, resulting in increased levels of protein expression within the cell.
Cells are usually collected 24-72 hours after transfection for research aimed at analyzing the transient expression of transfected genes. The optimal time interval depends on the cell type of the transferred gene, research objectives, and specific expression characteristics. Analysis of gene products may require isolation of RNA or protein for enzyme activity assays or immunoassays, and the method used to harvest the cells will depend on the final product analyzed.
We provide an unparalleled variety of transfection reagents to meet your different needs. For researchers who wish to establish transient protein production in mammalian and insect cells in their laboratories, we provide variety types of transient transfection reagents to meet your different needs.
When performing transient transfection, you can choose standard or reverse transfection protocols.