* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

Non-small cell lung cancer (NSCLC), which accounts for approximately 85 % of lung cancers, is one of the most common cancers globally, leading to cancer-related death. NCI-H1975 cell, also known as H1975 and NCIH1975 cell, is a cell line established in July 1988 from a woman with no smoking history. NCI-H1975 cells are epithelial-like in morphology and have the characteristics of adherent growth. NCI-H1975 have been used in analytical studies of new drugs for lung cancer.

BOC Sciences offers reagents specifically formulated and optimized for the transfection of NCI-H1975 cells.

Product Information

Features

• Unique formulation-maximizes transfection performance of NCI-H1975 cells.
• Invisible cytotoxicity.
• Effective and robust cell delivery.
• High efficiency for small RNA (siRNA, shRNA, miRNA), mRNA, pDNA transfection.
• Reagents showed at least 90% transfection efficiency for siRNA delivery. Transfection efficiency was determined by qRT-PCR.

Optimization of transfection

• The highest transfection efficiency is obtained by varying the density of NCI-H1975 cells and the amount of transfection reagent.
• The high passage rate of NCI-H1975 cells and the use of antibiotics (or growth factors) may require a larger volume of NCI-H1975 transfection reagent in each reaction.

Reference

1. Tang ZH; et al. Characterization of osimertinib (AZD9291)-resistant non-small cell lung cancer NCI-H1975/OSIR cell line. Oncotarget. 2016: 7(49): 81598-81610.

Related Sections:

• A549 Transfection
• NCI-H460 Transfection
• NCI-H1299 Transfection