* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

NCI-H1299, also known as H1299 or CRL-5803, is commonly used as a transfected host for various biomedical applications. NCI-H1299 cells exhibit an epithelial cell morphology and produce neurofibrillary. Furthermore, NCI-H1299 cells are positive for keratin and poikilitic, but negative for neurofilaments. Similar to other immortalized cell lines, NCI-H1299 cells lack p53 protein expression. Thus they can divide and proliferate indefinitely. Studies have shown that NCI-H1299 cells can synthesize NMB protein but not gastrin-releasing peptide (GRP). Therefore, NCI-H1299 cells are suitable to use in lung cancer research. In BOC Sciences, we offer transfection reagents for the study of NCI-H1299 cell lines to our customers.

Product Information

Features

• Lower cytotoxicity.
• Extend RNAi application with optimized reagents and simultaneous delivery of siRNA and plasmids.
• Reproducible infection results.
• Suitable for standard reverse infection and high-throughput applications.

Transfection optimization

• Maximum transfection efficiency can be obtained by varying the density of NCI-H1299 cells.
• Vary the amount of transfection reagent to optimize transfection conditions.

References

1. Listed N; et al. NCI-navy medical oncology branch cell line. Journal of Cellular Biochemistry Supplement. 1996, 24: 1.
2. Giaccone G; et al. Neuromedin B is present in lung cancer cell lines. Cancer Research. 1992, 52(9 Suppl): 2732s.
3. Chang C; et al. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. Journal of Biological Chemistry. 1996, 271(25): 14649-52.

Related Sections:

• A549 Transfection
• NCI-H460 Transfection
• NCI-H1975 Transfection