NCI-H1299 Transfection

* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

NCI-H1299, also known as H1299 or CRL-5803, is commonly used as a transfected host for various biomedical applications. NCI-H1299 cells exhibit an epithelial cell morphology and produce neurofibrillary. Furthermore, NCI-H1299 cells are positive for keratin and poikilitic, but negative for neurofilaments. Similar to other immortalized cell lines, NCI-H1299 cells lack p53 protein expression. Thus they can divide and proliferate indefinitely. Studies have shown that NCI-H1299 cells can synthesize NMB protein but not gastrin-releasing peptide (GRP). Therefore, NCI-H1299 cells are suitable to use in lung cancer research. In BOC Sciences, we offer transfection reagents for the study of NCI-H1299 cell lines to our customers.

Product Information

Catalog Number BT-000047
Contents Includes NCI-H1299 transfection reagent and transfection enhancer.
Storage Store at 4℃. Appropriate storage of the reagent is stable for up to 6 months.
Shipping NCI-H1299 transfection reagent supplies in liquid form and ships at ambient temperature.
Transfection Efficiency At least 90% efficiency of siRNA delivery. Transfection efficiency determines by qRT-PCR.
Use Limitations For research use only.


  • Lower cytotoxicity.
  • Extend RNAi application with optimized reagents and simultaneous delivery of siRNA and plasmids.
  • Reproducible infection results.
  • Suitable for standard reverse infection and high-throughput applications.

Transfection optimization

  • Maximum transfection efficiency can be obtained by varying the density of NCI-H1299 cells.
  • Vary the amount of transfection reagent to optimize transfection conditions.


  1. Listed N; et al. NCI-navy medical oncology branch cell line. Journal of Cellular Biochemistry Supplement. 1996, 24: 1.
  2. Giaccone G; et al. Neuromedin B is present in lung cancer cell lines. Cancer Research. 1992, 52(9 Suppl): 2732s.
  3. Chang C; et al. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. Journal of Biological Chemistry. 1996, 271(25): 14649-52.

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