* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.
Transfection, the process of introducing nucleic acids into eukaryotic cells, is a critical step in cell biology, gene expression, and gene suppression experiments. Transfection is the process of artificially introducing nucleic acid (DNA or RNA) into a cell using methods other than viral infection. The introduction of exogenous nucleic acids using a variety of chemical, biological or physical methods will alter the properties of the cell, enabling studies of cellular gene function and protein expression.
The mammalian cell expression system can facilitate the correct folding of proteins and achieve complex modifications, resulting in proteins that are closer to their natural state and can be used in areas with high activity requirements such as pharmaceuticals. There are usually two ways to produce proteins using mammalian cell expression systems: transient transfection and stable transfection.
The plasmids used for transient transfection and stable transfection are different. The plasmids used for transient transfection do not need to have resistance, while the plasmids used for stable transfection must have a specific resistance to facilitate subsequent clone screening. In addition, the medium and experimental reagents used in the two are also different.
The operation of transient transfection is simpler than that of constructing stable cell lines. After the plasmid is constructed, the target protein can be obtained through steps such as cell recovery, transfection, cell culture, and protein purification. In contrast, the construction of a stable cell line requires the linearisation of the constructed plasmid and then its introduction into cultured mammalian cells. Fusion of the plasmid to the cells is achieved by certain transfection methods, followed by cell pool screening, monoclonal screening and cell passaging to obtain a stably transfected cell line. The culture of the stable cell lines allows for long-term stable production of the target protein.
| Product Information | ||||
|---|---|---|---|---|
| Product Name | CRISPR RNP Transfection Reagent | Cas9 Transfection Reagent | Pro-Quick Transfection Reagent | Protein Transfection Reagent |
| Catalog Number | BT-000046 | BT-000005 | BT-000039 | BT-000038 |
| Appearance | Colorless. | Colorless liquid. | Colorless. | Colorless. |
| Application | CRISPR/Cas9 gene editing. | CRISPR/Cas9 gene editing. | Suitable for protein, peptide, antibody delivery. | Suitable for protein, peptide, antibody delivery. |
| Shelf Life | 1 year. | 1 year. | 1 year. | 1 year. |
| Storage | Store at 2-8°C. | Store at 2-8°C. Don't freeze! | Store at -20°C. | Store at 2-8°C. |
