TIB-152 Electroporation Kit (BT-000037)

* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

Product Information
Catalog Number BT-000037
Appearance Colorless liquid.
Application For delivery of plasmid DNA, siRNA, mRNA, and proteins.
Shelf Life 6 months.
Storage Store at 4°C. If stored properly, all components are stable for 6 months.
Brief Introduction TIB-152 cell is a suspension cell line synonymous with Jurkat, Clone E6-1, FHCRC. In the late 1970s, Schneider et al. discovered the TB-152 cell line by isolating peripheral blood from a 14-year-old boy diagnostic with T-cell leukemia and was named JM initially. The TIB-152 cell line produces interleukin 2, and its derivatives include D1.1, JCaM1.6, J.gamma-1, J.RT3-T3.5, I 2.1, and I 9.2 cell lines. TIB-152 cells are used to study acute T cell leukemia, T cell signaling, and various chemokine receptors susceptible to virus entry, especially the immortalized human T lymphocyte line expressing HIV. Furthermore, it has an extensive range of applications in biological research, such as the application of Jurkat cells in studying the ribonuclease P's M1-RNA, and the exploration of M1-RNA of the anti-MHC class II molecule transcriptional activator (CIITA) to inhibit MHC class II molecules expression on the cellular surface.
BOC Sciences' TIB-152 electroporation kit adopts the proprietary electroporation buffer formulation that simulates the cytoplasmic composition of TIB-152 cells and achieves effective transfection.
Cell Types TIB-152 cell.
Characteristics
  • Proprietary electroporation buffer formulation mimics cytoplasmic composition.
  • Efficient electroporation delivery and high cell viability provide reproducible electroporation results.
  • High cell viability and excellent cell recovery rate.
  • Certified electroporation buffer without RNAse and DNAse.
  • Provide pre-optimized electroporation protocols for delivering plasmid DNA, siRNA, mRNA and protein.
  • 75-90% siRNA delivery efficiency.
Contents Electroporation buffer, optimized electroporation solution for TIB-152.
Details The TIB-152 (Jurkat) cell line is commonly used to study leukemia, providing a model for studying T cell signaling, the expression of chemokine receptors that are susceptible to viral entry, and the effects of radiation and drugs. The BOC Sciences TIB-152 electroporation kit uses a proprietary electroporation buffer formulation that can simulate the cytoplasmic composition of TIB-152 cells to achieve effective transfection.
Electroporation Efficiency 75-90% efficiency for siRNA.
Form Colorless liquid.
Identification RNase and DNAse-free.
Note For rearch use only.
Shipping Transport at room temperature.
Use Limitations For rearch use only.
Description Designed for simple, high-efficiency TIB-152 electroporation.
References 1.Abraham BJ; et al. Small genomic insertions form enhancers that misregulate oncogenes. Nat Commun. 2017 Feb 9; 8: 14385.

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