* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.
|Application||For delivery of plasmid DNA, siRNA, mRNA, and proteins.|
|Shelf Life||6 months.|
|Storage||Store at 4°C. If stored properly, all components are stable for 6 months.|
|Brief Introduction||TIB-152 cell is a suspension cell line synonymous with Jurkat, Clone E6-1, FHCRC. In the late 1970s, Schneider et al. discovered the TB-152 cell line by isolating peripheral blood from a 14-year-old boy diagnostic with T-cell leukemia and was named JM initially. The TIB-152 cell line produces interleukin 2, and its derivatives include D1.1, JCaM1.6, J.gamma-1, J.RT3-T3.5, I 2.1, and I 9.2 cell lines. TIB-152 cells are used to study acute T cell leukemia, T cell signaling, and various chemokine receptors susceptible to virus entry, especially the immortalized human T lymphocyte line expressing HIV. Furthermore, it has an extensive range of applications in biological research, such as the application of Jurkat cells in studying the ribonuclease P's M1-RNA, and the exploration of M1-RNA of the anti-MHC class II molecule transcriptional activator (CIITA) to inhibit MHC class II molecules expression on the cellular surface.|
BOC Sciences' TIB-152 electroporation kit adopts the proprietary electroporation buffer formulation that simulates the cytoplasmic composition of TIB-152 cells and achieves effective transfection.
|Cell Types||TIB-152 cell.|
|Contents||Electroporation buffer, optimized electroporation solution for TIB-152.|
|Details||The TIB-152 (Jurkat) cell line is commonly used to study leukemia, providing a model for studying T cell signaling, the expression of chemokine receptors that are susceptible to viral entry, and the effects of radiation and drugs. The BOC Sciences TIB-152 electroporation kit uses a proprietary electroporation buffer formulation that can simulate the cytoplasmic composition of TIB-152 cells to achieve effective transfection.|
|Electroporation Efficiency||75-90% efficiency for siRNA.|
|Identification||RNase and DNAse-free.|
|Note||For rearch use only.|
|Shipping||Transport at room temperature.|
|Use Limitations||For rearch use only.|
|Description||Designed for simple, high-efficiency TIB-152 electroporation.|
|References||1.Abraham BJ; et al. Small genomic insertions form enhancers that misregulate oncogenes. Nat Commun. 2017 Feb 9; 8: 14385.|