* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.
|Application||Delivery of plasmids (DNA), siRNA, mRNA, and proteins.|
|Shelf Life||6 months.|
|Storage||Store at 4°C. Appropriate storage of reagent is stable for up to 6 months.|
|Brief Introduction||The CCL-246 (KG-1) cell line was obtained by H.P. Koeffler and D.W. Golde from a 59-year-old white male diagnosed with red acute myeloid leukemia (blood or bone marrow cancer). CCL-246 cells spontaneously differentiate into granulocytes and macrophage-like cells and express HLA A30, A31, B35, and Cw4 antigens. This cell line is EBNA negative (EBNA-) and demonstrated well responses to colony-stimulating factor (CSF). This cell line is used in cancer biomedical research, particularly in bone marrow and leukemia.
Transfection for suspension cell lines (e.g., CL-246 cells) has always been a challenging task routinely for laboratory research, as well as tissue engineering and gene therapy. Most research assays that work with adherent cell lines do not support suspension cell line transfection.
BOC Sciences' CCL-246 Electroporation Kit is a novel and unique chemical preparation specially formulated and optimized for CCL-246 cell line transfection. The proprietary formula ensures the highest transfection efficiency and viability of CCL-246 cells.
|Cell Types||CL-246 (KG-1) cell line.|
|Contents||Electroporation buffer, optimized electroporation solution for CCL-246.|
|Details||The CCL-246 (KG-1) cell line is a myeloid leukemia cell with a muscle cell morphology and is commonly used in cancer biomedical research, especially in relation to bone marrow and leukemia. The BOC Sciences CCL-246 electroporation kit uses a proprietary electroporation buffer formulation to help deliver nucleic acids efficiently and reliably.|
|Electroporation Efficiency||75-90% efficiency for siRNA.|
|Identification||RNase and DNAse-free.|
|Note||For rearch use only.|
|Use Limitations||For rearch use only.|
|Description||Designed for simple, high-efficiency CCL-246 electroporation.|
|References||1. Mrózek K; et al. Molecular cytogenetic characterization of the KG-1 and KG-1a acute myeloid leukemia cell lines by use of spectral karyotyping and fluorescence in situ hybridization. Genes Chromosomes Cancer. 2003 Nov; 38(3): 249-52.|