HEK293-Transfection Reagent (BT-000015)

* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

Product Information
Catalog Number BT-000015
Application Protein expression.
Shelf Life If stored properly, all components are stable for 6 months.
Storage Store at 2-8°C. If stored properly, all components are stable for 6 months.
Brief Introduction HEK293 cell line was first discovered in 1977, derived from human embryonic kidney cells transformed with sheared adenovirus 5 DNA grown in tissue culture. HEK293 has become a model cell line for researches due to its relatively easy maintenance and suitability for various transfection methods. It is now widely used for recombinant protein expression and virus production, and the study of gene function, gene overexpression, or gene knockdown is usually verified in HEK293 cells. In addition, recombinant HEK has also made significant contributions to drug screening, development, and biomanufacturing. Therefore, it is essential to effectively transfer genes into HEK293 for researches.
BOC Sciences HEK293-Transfection Reagent is a unique formulation based on polycationic liposomes of non-animal origin. It is suitable for large-scale transient transfection of HEK293 cells to produce recombinant proteins, monoclonal antibodies, viral particles (AAV, adenovirus, lentivirus), and virus-like particles.
Cell Types HEK293 cells, especially in suspension culture.
Characteristics
  • Stable and transient transfection.
  • Single or multiple plasmid delivery - ideal for virus production.
  • Compatible with serum-containing and serum-free media.
  • Greater than 70% transfection efficiency.
  • Low cytotoxicity, reproducible and easy to handle, no need for complex removal nor media replacement after transfection.
Contents Including HEK293 transfection reagent and transfection enhancer.
Details BOC Sciences HEK293-Transfection Reagent is a unique formulation based on polycationic liposomes of non-animal origin. It is designed for the transfection of HEK293 cells grown in suspension culture and is ideal for mammalian protein production.
Form Liquid.
Identification Without nuclease contamination and microbial contamination.
Note Toxicity: Flammable (J).
Shipping Wet ice.
Use Limitations For research use only.
Description Reagents for DNA delivery into HEK293.
References 1.Lorenzo E; et al. Plasticity of the HEK-293 cells, related to the culture media, as platform to produce a subunit vaccine against classical swine fever virus. AMB Express. 2019 Sep 5; 9(1): 139.
2.Böhm E; et al. Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells. BMC Biotechnol. 2015 Sep 18; 15: 87.

Related Products