293T/17 Transfection Reagent (BT-000016)

* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

Product Information
Catalog Number BT-000016
Shelf Life If stored properly, all components are stable for 6 months.
Storage Store at 4°C. If stored properly, all components are stable for 6 months.
Brief Introduction Kidney cancer is the 12th leading cause of death among cancer patients, with more than 338,000 new cases diagnosed yearly. Preclinical cell line models can support the discovery of novel treatments for kidney cancer patients. In 1973, the most primitive HEK293 cells were derived from the aborted human embryo's kidney cells. The human embryonic kidney cells were transformed into human type 5 adenovirus DNA fragments, resulting in the HEK293 cells. The HEK293T/17 cell line is a derivative of the HEK293T cell line. In detail, the HEK293 cell was co-transfected with pBND and pZAP plasmids, as well as the G418-resistant cell line to obtain HEK293T/17 cell line. This cell line not only retains the excellent performance of HEK293T cells but also has the characteristics of high transfection. Thus, the HEK293T/17 cell line can be used as a suitable host for kidney cancer research and kidney-related infection research. In short, HEK293T/17 can be used for various types of molecular and cell biology experimental research related to kidney research.
BOC Sciences provides high-efficiency transfection kits for the HEK293T/17 cell line. The 293T/17 Transfection Reagent is a non-animal source liposome-based reagent designed for efficient transfection of small RNA (siRNA, miRNA, shRNA, oligonucleotide) and DNA. The proprietary formulation of lipids and polymers ensures the maximum transfection efficiency and viability of HEK293T/17 cells.
Cell Types 293T/17 cells.
Characteristics
  • Proprietary cationic lipid formulation.
  • Optimized nucleic acid delivery to 293T/17 cells with reproducible results.
  • High transfection efficiency for small RNA (siRNA, shRNA, miRNA) and DNA.
  • Effective and robust intracellular delivery.
  • Transient or stable transfection.
  • Serums compatible.
  • Simplest and most straightforward protocol.
Contents Including 293T/17 transfection reagents and transfection enhancer reagent.
Details BOC Sciences 293T/17Transfection Reagent is an agent-based on liposomes of non-animal origin. It is designed for efficient transfection of small RNA (siRNA, miRNA, shRNA, oligonucleotide) and DNA.
Form Liquid.
Identification Without nuclease contamination and microbial contamination.
Shipping RT.
Transfection Efficiency At least 90% transfection efficiency of siRNA delivery.
Use Limitations For research use only.
Description Transfection reagent for 293T/17 Cells.
References 1.Hu J; et al. Human embryonic kidney 293 Cells: a vehicle for biopharmaceutical manufacturing, structural biology, and electrophysiology. Cells Tissues Organs. 2018; 205(1): 1-8.
2.Lin YC; et al. Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations. Nat Commun. 2014 Sep 3; 5: 4767.

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