Primary Cell Transfection Reagent (BT-000019)

* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

Product Information
Catalog Number BT-000019
Application Nucleic acids transiently or stably transfection in eukaryotic cells.
Storage Store at 2-8°C.
Brief Introduction Chromaffin cell is a kind of polygonal cell located in the medulla of mammalian adrenal gland. The cytoplasm of chromophores in the adrenal medulla contains a large number of chromophobic granules, which appear as vesicles of varying sizes under electron microscopy. Chromaffin cells are closely associated with the nervous system and are the most commonly studied neural crest derivatives. Chromaffin cells of the adrenal medulla contain and secrete dopamine (DA), epinephrine (E), noradrenaline (NE), neuropeptide Y (NPY), and peptides [Met] enkephalin (ME). Chromaffin cells are the primary activation site of the body's escape or combat responses. Moreover, human bodies circulate catecholamines (epinephrine, norepinephrine) and endorphins mainly from chromophores, which are stored in intracellular granules and released in response to stress. In addition to their advantages in cellular and molecular biology, chromophores are also valuable for stem cell research and clinical transplantation. BOC Sciences offers an advanced liposome-based unique formulation reagent designed explicitly for the transfection of chromaffin cells reagent that exhibits high sensitivity and low cytotoxicity.
Cell Types PC-12 cells.
Characteristics
  • Minimal cytotoxicity.
  • Excellent performance with high transfection efficiency and sufficiently gentle.
  • Fast and straightforward optimization protocols.
Contents Including chromaffin cells transfection reagents and transfection enhancer reagent.
Details BOC Sciences Primary Cell Transfection Reagent is a unique formulation based on liposomes specifically designed to transfect nucleic acids into primary cells. Under serum-free conditions, the primary cell transfection reagent and DNA can form a stable complex, which can be directly added to the cell culture medium and release the DNA into the cytoplasm by fusion with the cell membrane.
Features
  • Low cytotoxicity.
  • Suitable for stable and transient transfection.
  • Suitable for a wide range of primary cell types.
Form Liquid (aqueous solution).
Identification Without nuclease contamination and microbial contamination.
Note Do not freeze.
Shipping Shipped at room temperature.
Use Limitations For research use only.
Description Lipid reagent for transfecting sensitive and primary cells.
References 1.Eiden LE; et al. What's New in Endocrinology: The Chromaffin Cell. Front Endocrinol (Lausanne). 2018 Dec 4; 9: 711.

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