HepG2 Transfection Reagent (BT-000013)

* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.

Product Information
Catalog Number BT-000013
Shelf Life If stored properly, all components are stable for 6 months.
Storage Store at 4°C. If stored properly, all components are stable for 6 months.
Brief Introduction Liver cancer is considered the second leading cause of cancer-related deaths in the world. It has the characteristics of a high recurrence rate and low early detection rate, and it is a highly invasive and destructive disease. HepG2 is a human liver cancer cell line, most commonly used in drug metabolism and liver toxicity studies. HepG2 cells are non-tumor-causing cells with a high proliferation rate and epithelioid morphology, which can perform various differentiated liver functions. Originally, HepG2 was derived from the liver cancer tissue of a 15-year-old white man, and this cell secretes various plasma proteins, including serum proteins, alpha 2-macroglobulin, haemofibrinolytic plasminogen, iron transferrin, etc. A study has proved that HepG2 cells are resistant to G418. Moreover, HepG2 cells and their derivatives are used as a model system for studying liver metabolism and xenobiotic toxicity, antigen toxicity and genotoxic drugs, understanding the occurrence of liver cancer, detecting cell protection, and drug targeting research. Overall, this cell line is essential for biomedical researches.
BOC Sciences offers a unique transfection kit for HepG2 cell lines based on non-animal-derived cationic liposomes, ideal for in vitro delivery of DNA and RNA.
Cell Types HepG2 cell line.
Characteristics
  • Low cytotoxicity, using optimized cationic lipid formulations.
  • Suitable for transfection of small RNA (siRNA, shRNA, miRNA), mRNA, plasma DNA.
  • Repeatable and reliable results.
  • Serum compatible.
  • Can be used for stable cell line construction.
  • Effective and robust intracellular delivery.
Contents Including HepG2 transfection reagent and transfection enhancer.
Details BOC Sciences HepG2 Transfection Reagent is a unique formulation based on cationic liposomes of non-animal origin. It is designed to transfect HepG2 cell line and is ideal for in vitro delivery of DNA and RNA.
Form Liquid.
Identification Without nuclease contamination and microbial contamination.
Shipping Shipped at room temperature.
Transfection Efficiency At least 90% transfection efficiency.
Use Limitations For research use only.
Description Reagent designed for delivery of DNA and RNA into HepG2 cell line.
References 1.Donato MT; et al. Culture and Functional Characterization of Human Hepatoma HepG2 Cells. Methods Mol Biol. 2015; 1250: 77-93.

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