* This product is for research use only. Not intended for use in the treatment or diagnosis of disease.
| Product Information | |
|---|---|
| Catalog Number | BT-000005 |
| Appearance | Colorless liquid. |
| Application | CRISPR/Cas9 gene editing. |
| Storage | Store at 2-8°C. Don't freeze! |
| Brief Introduction | CRISPR-related protein is abbreviated as Cas. Clusters of regularly spaced short palindromic repeats (CRISPR) and related proteins (Cas) constitute the CRISPR-Cas system, which is the anti-phage immune system found in many bacteria and archaea. The most well-known Cas9 accounts for 2.59% of all Cas. The Cas9 domains are divided into REC domain, Ruvc domain, HNH domain, PI domain. Furthermore, the REC domain can be divided into REC1-A, REC1-B, REC2, and REC3 and serve as a connector for other domains. The Ruvc domain can be divided into three domains, which are not close to each other. Unlike CRISPR plasmids or Cas9 mRNA, the use of Cas9 protein provides excellent output efficiency in primary and stem cells. It eliminates the need of payloads for transcription or translation, minimizes potential risks related to genome integration, and is cell cycle independent. BOC Sciences' Cas9 Transfection Reagent is an optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 delivery. The Cas9 Transfection Reagent is gentler to cells and more cost-effective overall. |
| Cell Types | Common cells and primary cells. |
| Characteristics |
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| Contents | Including Cas9 transfection reagent and transfection enhancer. |
| Details | Unlike CRISPR plasmids or Cas9 mRNA, the use of Cas9 protein can provide excellent outting efficiency in primary cells and stem cells. It eliminates the need for transcription or translation of the payload, eliminates the risk of genome integration, and has nothing to do with the cell cycle. |
| Form | Colorless liquid. |
| Identification | Without nuclease contamination and microbial contamination. |
| Required Materials | CRISPR/Cas9 protein. |
| Shipping | RT or on wet ice. |
| Use Limitations | For research use only. |
| Description | Optimized lipid nanoparticle transfection reagent for CRISPR-Cas9 protein delivery |
| References | 1.Makarova KS; et al. Evolution and classification of the CRISPR-Cas systems. Nat Rev Microbiol. 2011 Jun; 9(6): 467-77. 2.Anders C; et al. Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease. Nature. 2014 Sep 25; 513(7519): 569-73. |